e coli o157 h7 atcc43889 Search Results


e coli o157 h7 strain atcc 43889  (ATCC)
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ATCC e coli o157 h7 strain atcc 43889
E Coli O157 H7 Strain Atcc 43889, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e coli o157 h7 atcc 43888  (ATCC)
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ATCC e coli o157 h7 atcc 43888
FIG. 2. CPRINS-FISH of the ampR gene in fixed cells of <t>E.</t> <t>coli</t> JM109 pUC19 (A and D) and E. coli <t>O157:H7</t> ATCC 43888 (C and F) and a fixed-cell mixture of E. coli JM109 pUC19 and E. coli O157:H7 ATCC 43888 (B and E). (A, B, and C) Under UV excitation, all DAPI-stained cells were visualized. (D, E, and F) Under green excitation, only cells with ampR gene-amplified products emitted red fluorescence of Alexa Fluor 546-labeled probe.
E Coli O157 H7 Atcc 43888, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e coli o157 h7 atcc 43889  (ATCC)
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ATCC e coli o157 h7 atcc 43889
Occurrence and levels of different microbial parameters in the Llobregat river a
E Coli O157 H7 Atcc 43889, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e coli o157 h7  (ATCC)
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ATCC e coli o157 h7
Occurrence and levels of different microbial parameters in the Llobregat river a
E Coli O157 H7, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e coli o157 h7 strains atcc 43889  (ATCC)
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ATCC e coli o157 h7 strains atcc 43889
FIGURE 1. Production of AI-2–like activity by various E. coli <t>O157:H7</t> strains (A) and time profiles of AI-2–like activity pro- duced by E. coli O157:H7 strains ATCC 43889 (closed symbols) and ATCC 43894 (open symbols) during 24 h of growth. AI-2– like activity in the culture fluids was determined by monitoring the induction of V. harveyi BB170 in a AI-2 bioassay.
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escherichia coli o157 h7  (ATCC)
96
ATCC escherichia coli o157 h7
FIGURE 1. Production of AI-2–like activity by various E. coli <t>O157:H7</t> strains (A) and time profiles of AI-2–like activity pro- duced by E. coli O157:H7 strains ATCC 43889 (closed symbols) and ATCC 43894 (open symbols) during 24 h of growth. AI-2– like activity in the culture fluids was determined by monitoring the induction of V. harveyi BB170 in a AI-2 bioassay.
Escherichia Coli O157 H7, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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media e coli o157 h7  (ATCC)
94
ATCC media e coli o157 h7
FIGURE 1. Production of AI-2–like activity by various E. coli <t>O157:H7</t> strains (A) and time profiles of AI-2–like activity pro- duced by E. coli O157:H7 strains ATCC 43889 (closed symbols) and ATCC 43894 (open symbols) during 24 h of growth. AI-2– like activity in the culture fluids was determined by monitoring the induction of V. harveyi BB170 in a AI-2 bioassay.
Media E Coli O157 H7, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stx2 o157 h7  (ATCC)
96
ATCC stx2 o157 h7
Specificity of SYBR Green PCR assay to detect <t> stx1 </t> and/or <t> stx2 </t> of various EHEC serotypes based on T m values a
Stx2 O157 H7, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIG. 2. CPRINS-FISH of the ampR gene in fixed cells of E. coli JM109 pUC19 (A and D) and E. coli O157:H7 ATCC 43888 (C and F) and a fixed-cell mixture of E. coli JM109 pUC19 and E. coli O157:H7 ATCC 43888 (B and E). (A, B, and C) Under UV excitation, all DAPI-stained cells were visualized. (D, E, and F) Under green excitation, only cells with ampR gene-amplified products emitted red fluorescence of Alexa Fluor 546-labeled probe.

Journal: Applied and Environmental Microbiology

Article Title: Recognition of Individual Genes in Diverse Microorganisms by Cycling Primed In Situ Amplification

doi: 10.1128/aem.71.11.7236-7244.2005

Figure Lengend Snippet: FIG. 2. CPRINS-FISH of the ampR gene in fixed cells of E. coli JM109 pUC19 (A and D) and E. coli O157:H7 ATCC 43888 (C and F) and a fixed-cell mixture of E. coli JM109 pUC19 and E. coli O157:H7 ATCC 43888 (B and E). (A, B, and C) Under UV excitation, all DAPI-stained cells were visualized. (D, E, and F) Under green excitation, only cells with ampR gene-amplified products emitted red fluorescence of Alexa Fluor 546-labeled probe.

Article Snippet: CPRINS-FISH of the ampR gene in fixed cells of E. coli JM109 pUC19 (A and D) and E. coli O157:H7 ATCC 43888 (C and F) and a fixed-cell mixture of E. coli JM109 pUC19 and E. coli O157:H7 ATCC 43888 (B and E). (A, B, and C) Under UV excitation, all DAPI-stained cells were visualized. (D, E, and F) Under green excitation, only cells with ampR gene-amplified products emitted red fluorescence of Alexa Fluor 546-labeled probe. on M arch 9, 2015 by G E O R G IA N C O U R T U N IV http://aem .asm .org/ D ow nloaded from Using combination of Sobr and other forward primers (JanSobf and Med-Sobf) or combination of Hydf and other reverse primers (Jan-Hydr and Sch-Hydr), the specificities of each CPRINS primer (Sobr and Hydf) were further tested with template DNAs from A. jandaei, A. media, and A. schubertii.

Techniques: Staining, Labeling

Occurrence and levels of different microbial parameters in the Llobregat river a

Journal:

Article Title: Comparative Survival of Free Shiga Toxin 2-Encoding Phages and Escherichia coli Strains outside the Gut

doi:

Figure Lengend Snippet: Occurrence and levels of different microbial parameters in the Llobregat river a

Article Snippet: E. coli O157:H7 ATCC 43889, which produces Stx2, and bacteriophage 933 W, obtained by mitomycin C lysogenic induction of E. coli strain C600(933W) ( 17 ), were used as positive controls.

Techniques:

FIGURE 1. Production of AI-2–like activity by various E. coli O157:H7 strains (A) and time profiles of AI-2–like activity pro- duced by E. coli O157:H7 strains ATCC 43889 (closed symbols) and ATCC 43894 (open symbols) during 24 h of growth. AI-2– like activity in the culture fluids was determined by monitoring the induction of V. harveyi BB170 in a AI-2 bioassay.

Journal: Journal of food protection

Article Title: Proteome analysis of virulence factor regulated by autoinducer-2-like activity in Escherichia coli O157:H7.

doi: 10.4315/0362-028x-70.2.300

Figure Lengend Snippet: FIGURE 1. Production of AI-2–like activity by various E. coli O157:H7 strains (A) and time profiles of AI-2–like activity pro- duced by E. coli O157:H7 strains ATCC 43889 (closed symbols) and ATCC 43894 (open symbols) during 24 h of growth. AI-2– like activity in the culture fluids was determined by monitoring the induction of V. harveyi BB170 in a AI-2 bioassay.

Article Snippet: Production of AI-2–like activity by various E. coli O157:H7 strains (A) and time profiles of AI-2–like activity produced by E. coli O157:H7 strains ATCC 43889 (closed symbols) and ATCC 43894 (open symbols) during 24 h of growth.

Techniques: Activity Assay, Bioassay

FIGURE 2. Effect of AI-2–like activity on the attachment of E. coli O157:H7 ATCC 43894 to HT-29 epithelial cells (A) (closed bars, control strain 43894; open bars, strain 43894 with AI-2– like activity). Fluorescence images of E. coli O157:H7 43894 ex- posed to AI-2–like activity on HT-29 cells (B).

Journal: Journal of food protection

Article Title: Proteome analysis of virulence factor regulated by autoinducer-2-like activity in Escherichia coli O157:H7.

doi: 10.4315/0362-028x-70.2.300

Figure Lengend Snippet: FIGURE 2. Effect of AI-2–like activity on the attachment of E. coli O157:H7 ATCC 43894 to HT-29 epithelial cells (A) (closed bars, control strain 43894; open bars, strain 43894 with AI-2– like activity). Fluorescence images of E. coli O157:H7 43894 ex- posed to AI-2–like activity on HT-29 cells (B).

Article Snippet: Production of AI-2–like activity by various E. coli O157:H7 strains (A) and time profiles of AI-2–like activity produced by E. coli O157:H7 strains ATCC 43889 (closed symbols) and ATCC 43894 (open symbols) during 24 h of growth.

Techniques: Activity Assay, Control, Fluorescence

FIGURE 3. Survival of C. elegans fed on E. coli O157:H7 ATCC 43894 in the presence of AI-2–like activity for 6 days. Closed circle, E. coli OP50 (laboratory food source); open circle, E. coli 43894 (control); triangle, E. coli 43894 (AI-2–like activity).

Journal: Journal of food protection

Article Title: Proteome analysis of virulence factor regulated by autoinducer-2-like activity in Escherichia coli O157:H7.

doi: 10.4315/0362-028x-70.2.300

Figure Lengend Snippet: FIGURE 3. Survival of C. elegans fed on E. coli O157:H7 ATCC 43894 in the presence of AI-2–like activity for 6 days. Closed circle, E. coli OP50 (laboratory food source); open circle, E. coli 43894 (control); triangle, E. coli 43894 (AI-2–like activity).

Article Snippet: Production of AI-2–like activity by various E. coli O157:H7 strains (A) and time profiles of AI-2–like activity produced by E. coli O157:H7 strains ATCC 43889 (closed symbols) and ATCC 43894 (open symbols) during 24 h of growth.

Techniques: Activity Assay, Control

FIGURE 4. Two-dimensional gel electro- phoresis images of protein extracts of E. coli O157:H7 ATCC 43894 exposed to control conditions (A) and AI-2–like activ- ity (B). The crude protein extracts (700 g) were separated on nonlinear immobi- lized pH gradient strips (pH 3.0 to 10.0) and electrophoresed on a 12.5% SDS- polyacrylamide gel. Outlined sections in- dicate zones presented in Figure 5.

Journal: Journal of food protection

Article Title: Proteome analysis of virulence factor regulated by autoinducer-2-like activity in Escherichia coli O157:H7.

doi: 10.4315/0362-028x-70.2.300

Figure Lengend Snippet: FIGURE 4. Two-dimensional gel electro- phoresis images of protein extracts of E. coli O157:H7 ATCC 43894 exposed to control conditions (A) and AI-2–like activ- ity (B). The crude protein extracts (700 g) were separated on nonlinear immobi- lized pH gradient strips (pH 3.0 to 10.0) and electrophoresed on a 12.5% SDS- polyacrylamide gel. Outlined sections in- dicate zones presented in Figure 5.

Article Snippet: Production of AI-2–like activity by various E. coli O157:H7 strains (A) and time profiles of AI-2–like activity produced by E. coli O157:H7 strains ATCC 43889 (closed symbols) and ATCC 43894 (open symbols) during 24 h of growth.

Techniques: Two-Dimensional Gel Electrophoresis, Control

FIGURE 5. Enlarged partial two-dimen- sional images representing crude protein extracts of E. coli O157:H7 ATCC 43894 exposed to control conditions (A) and AI- 2–like activity (B). Circled and boxed pro- tein spots indicate proteins that are up- and down-regulated by AI-2–like activity compared with controls, respectively. Marked protein spots were identified by MALDI-TOF-MS (see Table 2).

Journal: Journal of food protection

Article Title: Proteome analysis of virulence factor regulated by autoinducer-2-like activity in Escherichia coli O157:H7.

doi: 10.4315/0362-028x-70.2.300

Figure Lengend Snippet: FIGURE 5. Enlarged partial two-dimen- sional images representing crude protein extracts of E. coli O157:H7 ATCC 43894 exposed to control conditions (A) and AI- 2–like activity (B). Circled and boxed pro- tein spots indicate proteins that are up- and down-regulated by AI-2–like activity compared with controls, respectively. Marked protein spots were identified by MALDI-TOF-MS (see Table 2).

Article Snippet: Production of AI-2–like activity by various E. coli O157:H7 strains (A) and time profiles of AI-2–like activity produced by E. coli O157:H7 strains ATCC 43889 (closed symbols) and ATCC 43894 (open symbols) during 24 h of growth.

Techniques: Control, Activity Assay

Specificity of SYBR Green PCR assay to detect stx1 and/or stx2 of various EHEC serotypes based on T m values a

Journal:

Article Title: Rapid Detection of Escherichia coli O157:H7 with Multiplex Real-Time PCR Assays

doi: 10.1128/AEM.68.6.3169-3171.2002

Figure Lengend Snippet: Specificity of SYBR Green PCR assay to detect stx1 and/or stx2 of various EHEC serotypes based on T m values a

Article Snippet: The supernatant (1 μl) was added directly to the PCR mixture. table ft1 table-wrap mode="anchored" t5 caption a7 E. coli strain (strain number or CRIFS b Culture collection) Sorbitol Shiga toxin phenotype T m (°C) of: stx1 stx2 O157:H7 (ATCC 43890) − Stx1 82.49 O157:H7 (ATCC 43889) − Stx2 84.03 O157:H7 (C9490) − Stx1 and Stx2 82.03 84.05 O157:H7 (ATCC 43888) − O157:H7 JG1 − Stx1 and Stx2 81.74 83.88 O157:H7 JG2 − Stx1 and Stx2 81.70 83.91 O157:H7 JG3 − Stx2 83.46 O157:H7 JG4 − Stx2 83.53 O157:H7 JG5 − Stx1 and Stx2 81.67 83.86 O157:H7 JG6 − Stx1 and Stx2 81.62 83.73 O157:H7 JG7 − Stx1 and Stx2 81.45 83.60 O157:H7 JG8 − Stx1 and Stx2 81.49 83.65 O157:H7 JG9 − Stx1 and Stx2 81.54 83.66 O157:H7 JG10 − Stx2 83.31 O157:H7 JG11 − Stx1 and Stx2 81.65 84.0 O157:H7 JG12 − Stx1 and Stx2 81.42 83.76 O157:H7 JG13 − Stx1 and Stx2 81.40 83.78 O157:H7 JG14 − Stx1 and Stx2 81.47 83.77 O157:H7 JG15 − Stx1 and Stx2 81.48 83.78 O157:NM JG16 − Stx2 83.34 O157:H7 JG17 − Stx1 and Stx2 82.5 84.55 O157:H7 JG18 − Stx1 and Stx2 82.5 84.55 O157:H7 JG19 − Stx1 and Stx2 82.5 84.4 O157:H7 JG20 − Stx1 and Stx2 82.61 84.0 O2:H6 JG21 + O2:H8 JG22 + O103:H2 JG23 + Stx1 81.91 O26:H11 JG24 + Stx1 81.84 O91:H21 JG25 + Stx2 84.16 O45:H2 JG26 + Stx1 82.13 O111:H8 JG27 + Stx1 82.01 O26:H11 JG28 + Stx1 82.10 O91:H21 JG29 + Stx2 83.97 O103:H2 JG30 + Stx1 81.90 O111:H8 JG31 + Stx1 81.89 O111:H− JG32 + Stx1 and Stx2 81.28 83.73 O113:H21 JG33 + Stx1 and Stx2 81.27 83.70 ATCC 15597 + Open in a separate window a The presence of stx1 was further confirmed with the PCR primer pair designed in the present study.

Techniques: SYBR Green Assay

Primers for detection of E. coli O157:H7

Journal:

Article Title: Rapid Detection of Escherichia coli O157:H7 with Multiplex Real-Time PCR Assays

doi: 10.1128/AEM.68.6.3169-3171.2002

Figure Lengend Snippet: Primers for detection of E. coli O157:H7

Article Snippet: The supernatant (1 μl) was added directly to the PCR mixture. table ft1 table-wrap mode="anchored" t5 caption a7 E. coli strain (strain number or CRIFS b Culture collection) Sorbitol Shiga toxin phenotype T m (°C) of: stx1 stx2 O157:H7 (ATCC 43890) − Stx1 82.49 O157:H7 (ATCC 43889) − Stx2 84.03 O157:H7 (C9490) − Stx1 and Stx2 82.03 84.05 O157:H7 (ATCC 43888) − O157:H7 JG1 − Stx1 and Stx2 81.74 83.88 O157:H7 JG2 − Stx1 and Stx2 81.70 83.91 O157:H7 JG3 − Stx2 83.46 O157:H7 JG4 − Stx2 83.53 O157:H7 JG5 − Stx1 and Stx2 81.67 83.86 O157:H7 JG6 − Stx1 and Stx2 81.62 83.73 O157:H7 JG7 − Stx1 and Stx2 81.45 83.60 O157:H7 JG8 − Stx1 and Stx2 81.49 83.65 O157:H7 JG9 − Stx1 and Stx2 81.54 83.66 O157:H7 JG10 − Stx2 83.31 O157:H7 JG11 − Stx1 and Stx2 81.65 84.0 O157:H7 JG12 − Stx1 and Stx2 81.42 83.76 O157:H7 JG13 − Stx1 and Stx2 81.40 83.78 O157:H7 JG14 − Stx1 and Stx2 81.47 83.77 O157:H7 JG15 − Stx1 and Stx2 81.48 83.78 O157:NM JG16 − Stx2 83.34 O157:H7 JG17 − Stx1 and Stx2 82.5 84.55 O157:H7 JG18 − Stx1 and Stx2 82.5 84.55 O157:H7 JG19 − Stx1 and Stx2 82.5 84.4 O157:H7 JG20 − Stx1 and Stx2 82.61 84.0 O2:H6 JG21 + O2:H8 JG22 + O103:H2 JG23 + Stx1 81.91 O26:H11 JG24 + Stx1 81.84 O91:H21 JG25 + Stx2 84.16 O45:H2 JG26 + Stx1 82.13 O111:H8 JG27 + Stx1 82.01 O26:H11 JG28 + Stx1 82.10 O91:H21 JG29 + Stx2 83.97 O103:H2 JG30 + Stx1 81.90 O111:H8 JG31 + Stx1 81.89 O111:H− JG32 + Stx1 and Stx2 81.28 83.73 O113:H21 JG33 + Stx1 and Stx2 81.27 83.70 ATCC 15597 + Open in a separate window a The presence of stx1 was further confirmed with the PCR primer pair designed in the present study.

Techniques: Sequencing

Tm curve analysis of SYBR Green assay using real-time PCR. Tm was 81.8 and 83.8°C for stx1 (ATCC 43890) and stx2 (ATCC 43889), respectively. Sample 3 contained both stx1 and stx2 (C9490), and the Tm could be resolved between the two products. Sample 4 was a negative control (ATCC 43888).

Journal:

Article Title: Rapid Detection of Escherichia coli O157:H7 with Multiplex Real-Time PCR Assays

doi: 10.1128/AEM.68.6.3169-3171.2002

Figure Lengend Snippet: Tm curve analysis of SYBR Green assay using real-time PCR. Tm was 81.8 and 83.8°C for stx1 (ATCC 43890) and stx2 (ATCC 43889), respectively. Sample 3 contained both stx1 and stx2 (C9490), and the Tm could be resolved between the two products. Sample 4 was a negative control (ATCC 43888).

Article Snippet: The supernatant (1 μl) was added directly to the PCR mixture. table ft1 table-wrap mode="anchored" t5 caption a7 E. coli strain (strain number or CRIFS b Culture collection) Sorbitol Shiga toxin phenotype T m (°C) of: stx1 stx2 O157:H7 (ATCC 43890) − Stx1 82.49 O157:H7 (ATCC 43889) − Stx2 84.03 O157:H7 (C9490) − Stx1 and Stx2 82.03 84.05 O157:H7 (ATCC 43888) − O157:H7 JG1 − Stx1 and Stx2 81.74 83.88 O157:H7 JG2 − Stx1 and Stx2 81.70 83.91 O157:H7 JG3 − Stx2 83.46 O157:H7 JG4 − Stx2 83.53 O157:H7 JG5 − Stx1 and Stx2 81.67 83.86 O157:H7 JG6 − Stx1 and Stx2 81.62 83.73 O157:H7 JG7 − Stx1 and Stx2 81.45 83.60 O157:H7 JG8 − Stx1 and Stx2 81.49 83.65 O157:H7 JG9 − Stx1 and Stx2 81.54 83.66 O157:H7 JG10 − Stx2 83.31 O157:H7 JG11 − Stx1 and Stx2 81.65 84.0 O157:H7 JG12 − Stx1 and Stx2 81.42 83.76 O157:H7 JG13 − Stx1 and Stx2 81.40 83.78 O157:H7 JG14 − Stx1 and Stx2 81.47 83.77 O157:H7 JG15 − Stx1 and Stx2 81.48 83.78 O157:NM JG16 − Stx2 83.34 O157:H7 JG17 − Stx1 and Stx2 82.5 84.55 O157:H7 JG18 − Stx1 and Stx2 82.5 84.55 O157:H7 JG19 − Stx1 and Stx2 82.5 84.4 O157:H7 JG20 − Stx1 and Stx2 82.61 84.0 O2:H6 JG21 + O2:H8 JG22 + O103:H2 JG23 + Stx1 81.91 O26:H11 JG24 + Stx1 81.84 O91:H21 JG25 + Stx2 84.16 O45:H2 JG26 + Stx1 82.13 O111:H8 JG27 + Stx1 82.01 O26:H11 JG28 + Stx1 82.10 O91:H21 JG29 + Stx2 83.97 O103:H2 JG30 + Stx1 81.90 O111:H8 JG31 + Stx1 81.89 O111:H− JG32 + Stx1 and Stx2 81.28 83.73 O113:H21 JG33 + Stx1 and Stx2 81.27 83.70 ATCC 15597 + Open in a separate window a The presence of stx1 was further confirmed with the PCR primer pair designed in the present study.

Techniques: SYBR Green Assay, Real-time Polymerase Chain Reaction, Negative Control